Table 1.

General health and neurological reflexes

Genotype	WT			App-KI
Anesthesia	Air	Desflurane	Sevoflurane	Air	Desflurane	Sevoflurane
General health check
Body weight (g)	33.0 ± 0.9	31.1 ± 0.8	30.3 ± 1.4	31.2 ± 0.6	30.6 ± 0.6	30.7 ± 0.9
Body size (% with average size)	106.2 ± 2.8	100.1 ± 2.6	97.6 ± 4.5	100.4 ± 2.0	98.4 ± 2.0	98.7 ± 2.8
Bald patch (% with)	28.6	25.0	50.0	0.0	8.3	0.0
Fur (% with normal fur)	100.0	62.5	50.0	85.7	83.3	100.0
Crustiness around the nostrils/eyes (% with)	0.0	0.0	0.0	0.0	0.0	0.0
Ear pinna/Footpad color (% with normal color)	100.0	100.0	100.0	100.0	100.0	100.0
Leisons on the feet/tail (% with)	14.3	0.0	0.0	0.0	0.0	0.0
Scabs on the tail, rump, back (% with)	0.0	0.0	0.0	0.0	0.0	0.0
Tumor (% with)	0.0	0.0	0.0	0.0	0.0	0.0
Gait (% with normal gate)	100.0	100.0	100.0	100.0	100.0	100.0
Posture (% with normal posture)	100.0	100.0	100.0	100.0	100.0	100.0
Whisker (% with normal whisker)	42.9	50.0	66.7	71.4	83.3	75.0
Neurological screening test
Ear twich (% with quick response)	100.0	100.0	100.0	100.0	100.0	100.0
Eye blink (% with normal response)	85.7	100.0	100.0	85.7	75.0	91.7
Whisker-touch (% with normal response)	42.9	50.0	66.7	71.4	75.0	75.0
Postual reflex (% with normal response)	100.0	100.0	100.0	100.0	100.0	91.7
Righting reflex (% with normal response)	100.0	100.0	100.0	100.0	100.0	100.0

The general health check indicated that there are no differences among each group [ps > 0.050], except bald patch [χ² (5) = 13.168, p = 0.022]. A post hoc analysis revealed that App-KI mice showed fewer bald patch than WT control [p = 0.001]. The neurological reflexes of all groups showed similar performances [ps > 0.050]. (WT-Air: N = 7, WT-Des: N = 8, WT-Sev: N = 6, App-Air: N = 14, App-Des: N = 12, App-Sev: N = 12). Values are mean ± SEM