Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 7;41:128. doi: 10.1186/s13046-022-02351-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Identification of mouse mesenchymal stem cells (mMSCs) and mMSCs-derived exosomes. A Flow cytometric analysis for cell surface markers of CD44, Sca-1, CD90.2, CD34, CD11b, and CD45 on mMSCs. Percentage of positive cells shown in upper right corner as standardized with the isotype control antibody-incubated cells. B Assessment of differentiating capacity of mMSCs into osteocytes, chondrocytes, and adipocytes stained by Alizarin Red (left), Alcian Blue (middle), and Oil Red (right), respectively. Scale bar = 100 μm. C Representative image of transmission electron microscopy of mMSCs-Exo, and red arrows indicate typical exosomes. Scale bar = 200 nm. D Western Blot analysis for exosomal markers CD63, TSG101, and CD9, as well as the negative marker GM130 in mMSCs-Exo. The culture medium was used as control. E Nanoparticle tracking analysis of mMSCs-Exo. F Confocal images shown that PKH67-labeled exosomes (green) were taken up by MLE-12 cells at indicated time points in vitro. Nuclei were counterstained with DAPI. Scale bar = 40 μm