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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Cell Stem Cell. 2022 Jan 24;29(2):315–327.e6. doi: 10.1016/j.stem.2021.12.011

Figure 6. Fibroblast-targeted lentiviral Trps1 overexpression and knockdown.

Figure 6.

(A) Gross photos, PBS-treated wounds at POD 30 (Col1a1-CreERT;Ai9 mice) injected with control lentivirus (Trps1-OEC, left), Trps1 OE lentivirus at POD −3/7 (Trps1-OEE, middle), or POD 7/17 (Trps1-OEL, right). Red dotted lines demarcate the region where the silicone splint was attached and later removed.

(B) H&E (top row) and IF (bottom row; GFP+Ai9+ cells, successfully transduced fibroblasts) histology of Trps1-OEC, Trps1-OEE, and Trps1-OEL wounds.

(C) IF staining for Trps1 in indicated wounds; white arrowheads, nuclear Trps1.

(D) Quantification of HF (left), glands (middle), and ECM score (right).

(E) Matrix ultrastructural analysis of UW skin and POD 30 Trps1-OEC, Trps1-OEE, and Trps1-OEL wounds.

(F–J) As in (A–E), but for verteporfin-treated wounds with control lentivirus (Trps1-KDC), Trps1 KD lentivirus at POD 3/7 (Trps1-KDE), or POD 7/17 (Trps1-KDL). In (D) and (I), error bars bars indicate mean ± standard error (SEM).