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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Ocul Surf. 2021 Jun 11;21:193–205. doi: 10.1016/j.jtos.2021.06.006

Fig. 2.

Fig. 2.

EphA2 regulates the generation of cilia: (A) Venn diagram showing the overlap of potential EphA2 regulated genes determined by RNAseq analysis in corneal epithelium and ciliogenesis related genes (cilia related genes that obtained from ciliaCarta and GO term cilium, cilium organization). DEG: differentially expressed genes. Representation factor indicates overlapping: a higher number means more overlapping. (B) Graph showing the percent of cells with cilia in HCEC and HLEC treated with siControl or siEphA2. Scattered dots: values for replica. Immunostaining of acetylated tubulin (red) and α-tubulin (green) in whole mount corneas of WT (C) or EphA2 KO (D) mice (14 day old). Below each image is an enlargement of the highlighted inset. White arrows in inset: cilia. DAPI was used to stain nuclei. (E) Graph showing number of cilia from (C) and (D). Scattered dots: values for an individual mouse. (F–I) Confocal microscopy of Xenopus skin stained with acetylated tubulin to detect the cilium within the epithelial cells after (F) control morpholino (MO), (G) a morpholino targeting endogenous EphA2, or (H) overexpressing frog EphA2 in Xenopus skin treated with a morpholino targeting endogenous EphA2. Graphs showing percentage of cells with cilia (I) from images (F–H).