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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Ocul Surf. 2021 Jun 11;21:193–205. doi: 10.1016/j.jtos.2021.06.006

Fig. 4.

Fig. 4.

EphA2 regulates end-stage autophagy: (A) Phase-contrast microscopy of hTCEpi cells after siControl or siEphA2 transfection with or without BafA1 treatment for 24hr. Graph shows number of cells with vacuoles. Three fields (299 μm × 237 μm) per well in each experiment were counted. Each experiment replicates included 3 technical replicates Scattered dots: values for replica. (B) Confocal imaging of LAMP1 (red) immunofluorescent staining in hTCEpi cells after siControl or siEphA2 transfection with or without BafA1 treatment (100 nM) for 24hr. Inset shows enlarged area with small vesicles. (C–E) Western blotting showing EphA2, LC3I and LC3II levels in hTCEpi cells (C), HLECs (D) or HCEC (E) after siControl or siEphA2 transfection with or without BafA1 treatment for 24hr. Graphs show the densitometry analysis of blots for LC3II levels. GAPDH was used as a loading control. * = p < 0.05. N.S. = non-significant. n = 3.