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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Reproduction. 2020 Nov;160(5):659–672. doi: 10.1530/REP-20-0066

Figure 1 A-C. Western blot analysis on the effect of metformin on pAMPK and AMPK abundance in granulosa cells.

Figure 1 A-C.

Granulosa cells from prehierarchical follicles (3–5 and 6–8 mm) and preovulatory follicles (9–12 mm) were dispersed into single-cell suspension and treated with 0, 1, 10, or 20 mM metformin for 60 min in the presence or absence of rhFSH (10 ng/ml) at 41°C. Cell lysates were prepared following treatment, and a Western blot analysis was performed under reducing conditions to quantify the abundance of pAMPK, AMPK, and β-actin. Control received no rhFSH and no metformin. β-actin is used as a protein loading control. A,B P < 0.05 by ANOVA; n=3 replicates of 4 broiler breeder hens each. Inset: Photograph of prehierarchical follicles and the most-recently recruited preovulatory follicle to denote the source of granulosa cell (open box).