Table 1:
The nucleotide sequences of the primers directed towards Gallus gallus mRNA targets used to amplify FSHR, STAR, CYP11A1, HSD3B1 and RPL19. The length of amplicons and GenBank accession number are also provided.
| GenBank | Product | |||
|---|---|---|---|---|
| Name | Sequence | Accession #; position | Length (bp) |
|
|
| ||||
| A) | FSHR | |||
| cFSHRFwd1 | 5’ -TTGTGATCATCTGCATCTGCTA-3’ | NM_205079 | 127 | |
| cFSHRRev1 | 5’-CAGAGGAAGTCTGTGAAGATCAAT-3’ | |||
| B) | STAR | |||
| cSTARFwd2 | 5’-CTGAGCAGCAGGGATTTATCA-3’ | NM_204686 | 149 | |
| cSTARRev2 | 5’-GACCTGGTTGATGATGGTCTTT-3’ | |||
| C) | CYP11A1 | |||
| cP450Fwd2 | 5’-AGCACTTCAAGGGACTGAGCTT-3’ | NM_001001756 | 163 | |
| cP450Rev2 | 5’-ACAAGAATGAGGTCGAACTTGGT-3’ | |||
| D) | HSD3B1 | |||
| cHSDFwd1 | 5’-AAGTCAACGTGACAGGTACTCAGA-3’ | NM_205118 | 142 | |
| cHSDRevl | 5’-AAGTCAACGTGACAGGTACTCAGA-3’ | |||
| E) | RPL19 | |||
| RPLl9Fwd | 5’-CCCAACGAGACCAACGAG-3’ | NM_001030929 | 130 | |
| RPLl9Rev | 5’-CCAAGGTGTTCTTCCTGCAT-3’ | |||