. Author manuscript; available in PMC: 2022 Apr 7.

Published in final edited form as: Reproduction. 2020 Nov;160(5):659–672. doi: 10.1530/REP-20-0066

Table 2:

Reagents used for Western blot analyses. All antibodies were purchased from Cell Signaling Technology (Danvers, MA) except anti-β-actin and anti- α-tubulin antibodies that were purchased from Millipore Sigma (Burlington, MA).

Target	Protein quantity used (μg)	Electrophoresis Gel type	Primary antibody
Target	Protein quantity used (μg)	Electrophoresis Gel type	Name	Cat#	Concentration used
pAMPK	15–40	10 % Bis-Tris NuPAGE Novex gels	Rabbit anti-phospho-AMPKα1/2 (Thr172)	2531	1: 750^‡ or 1:1000^{‡ ‡}
AMPK	15–40	10 % Bis-Tris NuPAGE Novex gels	Rabbit anti-AMPKα	2532	1: 750^‡ or 1:1000^{‡ ‡}
pERK	10	10% Bis-Tris NuPAGE Novex gels	Rabbit anti-phospho- p44/42 MAPK (ERK1/2) (Thr202/Tyr204)	9101	1:1000
ERK	10	10% Bis-Tris NuPAGE Novex gels	Rabbit anti-p44/42 MAPK (ERK1/2)	9102	1:1000
pACC	15–40	4–12% Bis-Tris NuPAGE Novex gels	Rabbit anti-phospho- ACC (Ser79)	3661	1: 750^‡ or 1:1000^{‡ ‡}
ACC	15–40	4–12% Bis-Tris NuPAGE Novex gels	Rabbit anti-ACC	3662	1: 750^‡ or 1:1000^{‡ ‡}
pAkt	15	10% Bis-Tris NuPAGE Novex gels	Rabbit anti-phospho-Akt (Ser473)	9271	1:1000
Akt	15	10% Bis-Tris NuPAGE Novex gels	Rabbit anti-Akt	9272	1:1000
β-actin	--	Membranes reprobed	Mouse anti-α-tubulin	T6199	0.7 μg/mL
α-tubulin	--	Membranes reprobed	Mouse anti-β-Actin	A5441	0.5 μg/mL

^‡

9–12 mm follicle granulosa cells

^‡‡

3–5 mm and 6–8 mm follicle granulosa cells