Figure 3.

Histopathological effects of STR exposure on jejunum and ileum in CDDP-induced renal injury model. Eight-week-old wild type male mice (n = 3–5) were orally preexposed to the vehicle or streptomycin (20 mg/mouse) for 24 h and then renal injury was induced with CDDP (20 mg/kg) via intraperitoneal administration for an additional 72 h. (A, B) Histological observation of hematoxylin and eosin (H&E)-stained sections of the small intestine [jejunum (A) and ileum (B)] [magnification 100× (upper) and 200× (lower) with scale bar(s): 100 μm]. The arrows indicate neutrophil infiltration, stars indicate crypts, and arrowheads indicate epithelial lining integrity. (C, D) Quantitative comparisons of villus length (left) and crypt length (right). The results are shown as a box plot with Tukey whiskers and the different letters (a–c) over each box represent significant differences between groups (p < 0.05) in the jejunum (C) and the ileum (D). (E, F) Quantitative pathological scoring of neutrophilic infiltration (left), epithelial lining and ulcers (middle), and crypt loss (right) in the jejunum (E) and the ileum (F). Results are representative of three independent experiments. The results are shown as a bar graph and the different letters over each bar represent significant differences between the groups using ANOVA with Newman–Keuls method as a post hoc ANOVA assessment (p < 0.05).