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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Glia. 2021 Feb 24;69(6):1563–1582. doi: 10.1002/glia.23979

Figure 2. TRPV4 is expressed in retinal microglia.

Figure 2.

(A) Representative images of TRPV4 IHC in dissociated microglia. Dissociated microglia could be distinguished from photoreceptors and RGCs (*) by morphology and expression of CX3CR1GFP/+. (B) Vertical section of mouse retina expressing TRPV4eGFP. Microglia are identifiable by morphology and localization to the retinal ganglion cell/nerve fiber and the inner and outer plexiform layers (C-D) Retinal wholemounts expressing TRPV4eGFP and immunolabeled for Iba1 (magenta; C-D, middle panels) revealed robust expression in both reactive (C) and quiescent (D) microglia. Scale bar is 20 μm in B-D; 10 μm in A. onl, outer nuclear layer; opl, outer plexiform layer, inl, inner nuclear layer, ipl, inner plexiform layer, gcl, ganglion cell layer.