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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Glia. 2021 Feb 24;69(6):1563–1582. doi: 10.1002/glia.23979

Figure 7. Calcium response induced by HTS stimulation requires TRPV4 activation and PLA2 signaling.

Figure 7.

Dissociated microglia loaded with Fura-2 AM. (A) Representative trace of the microglial response to 285 mOsm HTS. (B) Representative microglial response to 190 mOsm HTS alone (black trace), and 190 mOsm HTS + HC-06 (red trace). (C) Microglial response to 190 mOsm HTS (black trace) and the presence of the PLA2 blocker p-bromophenacylbromide (pBPB) (red trace). (D) Summary of calcium responses to 285 and 190 mOsm HTS. The TRPV4-dependence of the HTS response was tested with HC-06, and PLA2-dependence with cytochrome P450 inhibitor clotrimazole (Clz) and pBPB. Data are shown as Mean ± S.E.M. Statistical significance was determined with a one-way ANOVA with Dunnett’s test for multiple comparisons. * Statistical comparison to isotonic, # statistical comparison to HTS. **/## p < 0.01, ****/#### p < 0.0001