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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Glia. 2021 Feb 24;69(6):1563–1582. doi: 10.1002/glia.23979

Figure 9. Decreasing the osmolarity by 36.7% results in retraction of complex branches and is rescued by selective TRPV4 antagonists.

Figure 9.

(A) Representative microglia during isotonic (green) and hypotonic (magenta) stimuli. (B) Representative time lapse images of changing microglial processes during isotonic saline (white) or HTS (red). (C). Representative microglia during isotonic (green) or HTS after pretreatment with HC-06 (magenta). (D) Representative time lapse images of changing microglial processes during isotonic (white), pre-incubation with HC-06 (yellow) and HTS + HC-06 (red). Scale bar in A and C = 20 μm, B and D = 5 μm.