FIG. 4.

ELISA and Western blot analyses of secretion of TSST-1 in S. aureus MN8. ELISA and Western blot analysis were performed to examine the extracellular and intracellular presence of TSST-1 in the presence of various potential secretion inhibitors. Cells were grown in the presence of compounds at the concentrations designated. The mobility of TSST-1 in the Western blot is noted with an arrow, while the mobility of a non-TSST-1 protein nonspecifically recognized by the antibody is noted with an asterisk. In panel D, nonspecific recognition of a marker protein is apparent in the empty middle lane. Percent inhibition is defined as for growth of TSST-1 secretion over 6 h in comparison with an untreated control. Western blots were scanned using a GS-700 Imaging Densitometer (Bio-Rad Laboratories, Hercules, Calif.), and graphics were added with Adobe Photoshop 5.0. (A) cerulenin; (B) compound 3; (C) compound 4; (D) compound 5; (E) compounds 1 (left, Western blot; top, ELISA) and 2 (right, Western blot; bottom, ELISA).