Table 1.

Enzyme Inhibition Constant Data of RA from Binding DB

S. No	Target	Disease	IC50 (nM)	Assay description	Reference
1	HIV type 1 integrase	Acquired immuno-deficiency syndrome	4000	Inhibition of strand transfer activity of HIV-1 integrase	Mazumder et al. (1997)
2	Tyrosine-protein kinase Lck	Cancer, autoimmune disorders, chronic inflammatory diseases	24,000	Inhibition of binding to p56 Lck tyrosine kinase SH2 domain	Park et al. (2003)
3	Matrix metalloproteinase-2	Cancer, arthritis, skin aging, blood vessel impairment	27,200	Inhibition of gelatinolytic activity of MMP2 in rat lung homogenate after 40 min by SDS-PAGE preincubated for 30 min	Murata et al. (2009)
4	Aldose reductase	Diabetes	3910	Inhibition of aldose reductase	Koukoulitsa et al. (2010)
5	Tyrosinase	Skin melanization	250,000	Inhibition of mushroom tyrosinase	Fujimoto et al. (2010)
6	Transcription factor AP-1	Rheumatoid arthritis and cancer	16,200	Inhibition of transcription factor AP-1 binding to oligonucleotide containing TPA-responsive element in TPA-activated human HeLa cells after 1 h	Chen et al. (2014)
7	Interstitial collagenase	Rheumatoid arthritis, fibrotic disease	5600	Inhibition of human recombinant MMP1 catalytic domain using Dnp-Pro-beta-cyclohexyl-Ala-Gly-Cys(Me)-His-Lys-(Nma)-NH2 as substrate pre-incubated	Yuan et al. (2013)
8	Proto-oncogene tyrosine-protein kinase Src	Cancer	26,000	Inhibition of c-SRC SH2 domain	Sperl et al. (2009)
9	Estradiol 17-beta-dehydrogenase 2	Osteoporosis	3720	Inhibition of human 17beta-HSD2 expressed in HEK293 cell lysates incubated for 10 min using [2,4,6,7-3H]-estradiol and NAD + by scintillation	Vuorinen et al. (2017)
10	Beta amyloid A4 protein	Alzheimer’s disease	71,900	Inhibition of amyloid beta (1 to 42 residues) (unknown origin) aggregation after 24 h by thioflavin T assay	Kwon et al. (2015)
11	Reverse transcriptase	Acquired immuno-deficiency syndrome	100,000	Inhibition of HIV1 reverse transcriptase	Dubois et al. (2008)
12	Integrase	Acquired immuno-deficiency syndrome	63,500	Inhibition of HIV1 integrase using labelled oligonucleotide substrate in presence of bovine serum albumin by ELISA	Dubois et al. (2008)
13	Aldose reductase	Diabetes complications	3890	Inhibition of Rattus norvegicus (rat) lens aldose reductase	Jain et al. (2012)
14	Tyrosine-protein kinase Fyn	Neurodegenerative disease	1300	Inhibition of human Fyn expressed in Sf9 cells after 20 min by ELISA in presence of 1 umol/L ATP	Jelić et al. (2007)
15	Lipoxygenase	Cancer, asthma, inflammatory disease	1,150,000	Inhibition of soybean lipoxygenase in vitro. Binding energy and IC-50 calculated to detect inhibitory activity	Koukoulitsa et al. (2007)
16	MARK-4	Cancer	6200	Inhibition of MARK-4 validated in cell-free and cell-based enzyme assay. Increased apoptosis in MARK-4 overexpressing cancer cells	Anwar et al. (2020)
17	Glycogen Synthase kinase 3β	Alzheimer’s disease	135	Inhibition of GSK-3 β enzyme involved in several human diseases	Paudel et al. (2018)