FIGURE 1.

Bmi‐1‐RING1B and autophagy are negatively related to SA‐PCH. (A) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was conducted using differentially expressed genes (DEGs) in young (14‐week‐old) and old (18‐month‐old) mouse hearts. (B) Gene set enrichment analysis (GSEA) on autophagy signal pathway and ubiquitin‐mediated proteolysis. (C) Genes Correlation analysis using 12‐ and 18‐ month mouse hearts RNAseq count data. (D) Gene Ontology (GO) analysis and GSEA were processed using genes that were highly related to Bmi‐1. (E) Western blots of cardiac extracts of WT young (8‐week‐old) or aging (20‐month‐old) mice showing Bmi‐1, RING1B, LC3BII, Ang II, GATA4, ANP, BNP, NF‐κB‐p65 and p‐p65 (Ser536), IκB‐α and p‐IκB‐α (Ser32); β‐actin was the loading control. (F) Protein levels relative to β‐actin were assessed by densitometric analysis. Six mice per group were used for experiments. Values are mean ± SEM of six determinations per group, **p < .01, ***p < .001 compared with the WT young mice, unpaired t‐test for bar graphs