TABLE 2.
Isogenic laboratory strains and plasmids used in the studya
| S. aureus strains | Characterization | Notable property(-ies) | Origin or reference(s) | MIC (μg/ml) of ciprofloxacin |
|---|---|---|---|---|
| Laboratory | ||||
| ISP794 | 8325 pig-131 | Parent strain | 7 | 0.25 |
| SS1 | MT5 gyrA | Amino acid change in GyrA (S84L) | 1 | 0.25 |
| MT5224c4 | MT5 flqA (grlA542) | Amino acid change in GrlA (S80F) | 31, 52 | 2.0 |
| EN1252a | MT5 flqA(grlA542) gyrA Ω1051(Erm) Nov+ | Double Amino acid changes in GrlA (S80F) and GyrA (S84L) | 1 | 32.0 |
| RN6911 | RN6390B Δagr::tetM | agr locus has been replaced with tetM | 34 | ND |
| ALC 355 | Newman Δagr::tetM | Newman with Δagr::tetM from strain RN6911 | 58 | ND |
| CB3 | EN1252a Δagr::tetM | Double grlA gyrA mutant with Δagr::tetM from strain ALC355 | This work | 32.0 |
| Plasmids | ||||
| pFNBA6 | Cmr Ampr | pSB327 shuttle vector with fused luxAB and fnbA promoter | 14 | |
| pFNBB6 | CMr Ampr | pSB327 shuttle vector with fused luxAB and fnbB promoter | 14 |
a
Abbreviations: ND, not determined; Cm, chloramphenicol; Amp, ampicillin.