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. 2022 Mar 24;13:814692. doi: 10.3389/fmicb.2022.814692

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Copyright © 2022 Metzendorf, Lange, Lainer, Schlüter, Dittmann, Paul, Troitzsch and Sievers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western Blot analysis of secreted toxin A. (A) C. difficile strain 630 (left) and strain R20291 (right) were cultivated for 72 h in the presence of four different bile acids (CA, cholic acid; DCA, deoxycholic acid; CDCA, chenodeoxycholic acid; LCA, lithocholic acid) and without any stress conditions (untreated). Secreted proteins were extracted from cultivation supernatant and an amount of 150 μg was separated via SDS-PAGE (loading control in the bottom) to subsequently detect toxin A by Western Blot analysis (top). (B) Three biological replicates were analyzed and related to the untreated condition. Standard deviations are indicated.