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. 2022 Mar 24;13:814692. doi: 10.3389/fmicb.2022.814692

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Copyright © 2022 Metzendorf, Lange, Lainer, Schlüter, Dittmann, Paul, Troitzsch and Sievers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thin layer chromatography (TLC) of C. difficile lipid extracts. (A) Lipids of exponentially growing cells and (B) stationary phase cells under control conditions (untreated) and challenged with different bile acids (CA, cholic acid; DCA, deoxycholic acid; CDCA, chenodeoxycholic acid; LCA, lithocholic acid) were extracted and separated via TLC. Hydrophobic species were stained with phosphomolybdic acid. References of the four bile acids were separated on the right side of each TLC plate. Two additional biological replicates for exponentially growing cells and stationary phase cells are provided Supplementary Figure 3.