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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Curr Protoc Cell Biol. 2020 Jun;87(1):e107. doi: 10.1002/cpcb.107

Figure 4. Examples of distributions of data got after tracking objects in Kymolyzer.

Figure 4.

Histograms of motility data obtained by analyzing and collating data from 27 axons with a total of 413 discrete mitochondria are shown. During data collation, when the average motility per kymograph is considered, the data generally follows a Gaussian trend (A). Data samples showing normal or close to normal distribution can be analyzed with traditional t-tests and summarized by their mean or median. However, when plotted per mitochondrion (object-wise collation), the data does not follow a normal distribution (B). Data that are not normally distributed should be analyzed with non-parametric statistical tests.