TABLE 2.

Results of IEF, genotyping of β-lactamases, plasmid analysis, and PFGE

Resistance phenotype group and strain	Clinical isolate						Transconjugantc
	pI(s)	SHV	TEM	AmpC	Plasmid profile	PFGE pattern	pI	SHV	AmpC	Approx plasmid size (kb)
Group I
X386	8.2, 7.6	5			A	I	8.2	5		100
C097	8.2, 7.6, 5.4	5	1		B	II	8.2	5		100
Q381	8.2, 7.6, 5.4	5	1		C	III	8.2	5		70
T923a	8.2, 7.6, 5.4	5	1		D	IV
T386	8.2, 7.6, 5.4	12	1		E	V	8.2	12		70
R436	8.2, 7.6, 5.4	12	1		E	VI	8.2	12		100
S276	8.2, 7.6, 5.4	12	1		F	VII	8.2	12		100
T986	8.2, 7.6, 5.4	12	1		G	VIII	8.2	12		70
B262a	8.2, 7.6, 5.4	12	1		H	IX
T255a	8.2, 7.6, 5.4	12	1		I	X
V293	8.2, 7.6, 5.4	12	1		G	XI	8.2	12		70
D155	8.2, 7.6	12			J	XII	8.2	12		40
B657	8.2, 7.6	12			K	XIII	8.2	12		75
C106	8.2, 7.6	12			J	XIV	8.2	12		40
Group II
O574	8.3, 7.6, 5.4	11	1		L	XV	8.3			95
R549	8.3, 7.6, 5.4	11	1		M	XVI	8.3			125
Group III
W142	8.25, 7.6, 5.4	11	1	CMY-8	N	XVII	8.25		CMY-8	25
W580	8.25, 7.6, 5.4	11	1	CMY-8	N	XVII	8.25		CMY-8	25
T384	8.25, 7.6, 5.4	11	1	CMY-8	N	XVII	8.25		CMY-8	25
Group IV
O787	8.2, 7.6, 5.4	12	1	UDb	O	XVIII	8.2	12	UD	150

^aConjugation experiments with strains T923, B262, and T255 were not successful. 

^bUD, undetermined. 

^cAll transconjugates lacked TEM.