Fig 4. Confocal microscopy images of P. falciparum sporozoites bound by anti-CSP antibodies.

P. falciparum sporozoites were dissected from infected mosquito salivary glands and purified. The binding of anti-PfCSP mAbs specific to N-terminal region of PfCSP (humanized mAb 5D5), NANP repeats (mAb311), β-ctCSP (mAb1512), and α-ctCSP (mAb236) was observed using the secondary antibody goat-anti-human Alexa 488 (green). A polyclonal anti-HSP70 antibody targeting P. falciparum heat shock protein 70 was used as a positive control and stained with the secondary antibody, goat anti-mouse Rhodamine Red-X (red). The sporozoite nuclei were stained with DAPI (blue). The merged images are also displayed. Images were acquired using a Zeiss LSM880 with Airyscan Confocal Microscope.