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. 2022 Mar 24;11:e76003. doi: 10.7554/eLife.76003

Figure 2. ceh-34 is expressed in all pharyngeal neurons.

(A) ceh-33 and ceh-34 loci showing different alleles and fosmid reporters used in this study. (B) Expression of the ceh-34 CRISPR/Cas-9-engineered reporter allele ot903 over the course of development. ceh-33 fosmid reporter (wgIs575) shows expression in a subset of head muscle cells. (C) Pharynx organ selector pha-4 controls ceh-34 expression (as analyzed with the wgIs524 transgene). Animals were scored at the L1 stage. Presumptive ‘pharyngeal cells’ in pha-4 mutant are marked with a red pha-4 promoter fusion (stIs10077). Cells co-expressing ceh-34 and pha-4 were counted (yellow cells). ceh-34 expression in head muscle cells, marked with red asterisk, is not affected since they do not express pha-4.

Figure 2.

Figure 2—figure supplement 1. Evolution of Sine oculis orthologs in nematodes.

Figure 2—figure supplement 1.

(A) Phylogeny of ceh-33/34 gene duplication in nematodes. Phylogeny based on homeodomains. (B) Synteny of the ceh-33/ceh-34 locus across several Caenorhabditis species.
Figure 2—figure supplement 2. Expression of the organ selector pha-4.

Figure 2—figure supplement 2.

(A) pha-4 expression (stIs10077) is not affected in ceh-34(tm3733) mutants. Animals were scored at the L1 stage. Statistical analysis was performed using unpaired t-test. (B) pha-4 is continuously expressed in pharyngeal neurons. Expression of a CRISPR/Cas9-engineered pha-4 reporter allele (ot946) in green and a rab-3prom reporter (otIs355) in red in L1 (left), L3 (middle), and adult (right) animals. High magnifications of the anterior bulb provided in the insets at the bottom-left corner of the merged images. Arrows point to pharyngeal neurons expressing both pha-4 and rab-3.