Figure 4. ceh-34 affects the expression of receptors for neurotransmitters and neuropeptides.

(A) Representative pictures and quantification showing neurotransmitter receptor expression loss in ceh-34 mutants. Reporter genes used are transgenes glr-2 (ivIs26), mgl-1 (otIs341), and a CRISPR/Cas9-engineered reporter allele for ser-7 (syb4502). Animals were scored at the L1 stage. Statistical analysis was performed using Fisher’s exact test or chi-square test. N is indicated within each bar and represents number of neurons scored. (B) Representative pictures and quantification showing neuropeptide receptor expression loss in ceh-34 mutants. Reporter gene used is the CRISPR/Cas9-engineered reporter allele trhr-1 (syb4453). Animals were scored at the L1 stage, with a red pan-neuronal marker (otIs355) in the background to facilitate scoring. Number of “yellow“ cells (overlap of red pan-neuronal marker and green reporter) within the pharynx were counted. Statistical analysis was performed using unpaired t-test. (C) Circuit diagram summarizing the effect of ceh-34 on neurotransmitter and neuropeptide receptor expression. Nodes lose coloring when expression is affected in ceh-34 mutants (gray indicates partial effect; ser-7 and trhr-1 are colored white in all neurons since identity of neurons with partial effect is not known). See legend to Figure 3 for more information on circuit diagram features.