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. 2022 Mar 24;11:e76003. doi: 10.7554/eLife.76003

Figure 5. ceh-34 affects neuropeptidergic identity of pharyngeal neurons.

Figure 5.

(A) Representative pictures and quantification showing expression of 10 different neuropeptides is affected in ceh-34 mutants. Reporter genes used are transgenic reporters for flp-2 (ynIs57), flp-4 (ynIs30), flp-15 (ynIs45), flp-21 (ynIs80), nlp-3 (otIs695), nlp-8 (otIs711), and nlp-13 (otIs742) and CRISPR/Cas9-engineered reporter alleles for flp-5 (syb4513), flp-28 (syb3207), and trh-1 (syb4421). Animals were scored at the L1 stage. Statistical analysis was performed using Fisher’s exact test, chi-square test, or unpaired t-test. N is indicated within each bar and represents number of neurons scored. (B) Circuit diagram summarizing the effect of ceh-34 on neuropeptide expression. Nodes lose coloring when neuropeptide expression is affected in ceh-34 mutants (gray indicates partial effect; flp-5 and nlp-3 are colored white in all neurons since identity of neurons with partial effect is not known). See legend to Figure 3 for more information on circuit diagram features.