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. 2022 Mar 24;11:e76003. doi: 10.7554/eLife.76003

Figure 9. Limited involvement of eya-1 in pharyngeal neuron identity specification.

Figure 9.

(A) eya-1 is expressed in all pharyngeal neurons throughout the life of the worm. Images of L1 and adult worms showing co-localization of eya-1 expression (nIs352) with the pan-neuronal gene rab-3 (otIs355) in pharyngeal neurons. (B) eya-1 expression is regulated by ceh-34. Representative pictures and quantification are shown. Reporter gene is eya-1 (nIs352). Animals were scored at the L1 stage. Statistical analysis was performed using Fisher’s exact test. N is indicated within each bar and represents number of worms scored. (C) eya-1 mutant animals show defects in neurotransmitter identity specification. Representative images and quantification are shown for unc-17 (otIs661), eat-4 (otIs487), and tph-1 (otIs517). Animals were scored at the L4 stage. Statistical analysis was performed using Fisher’s exact test. N is indicated within each bar and represents number of neurons scored.