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. 2022 Mar 31;49(5):72. doi: 10.3892/ijmm.2022.5128

Figure 8.

Figure 8

Knocking down FUNDC1 expression weakens the promotion of hypoxia-induced proliferation, migration and odontoblastic differentiation. (A) Representative images of the wound healing assay (scale bar, 1 mm) and (B) percentages of wound closure from three independent experiments are quantified. (C) The number of migratory cells stained with crystal violet solution from three independent experiments was quantified. (D) Representative images of migratory cells are shown. Scale bar, 100 µm. (E) Viability of HDPCs was detected by Cell Counting Kit-8 analysis. (F) ALP staining and (G) activity in the normoxic, hypoxia + NC and hypoxia + siFUNDC1 groups on days 3. Scale bar, 200 µm. (H) Protein expression levels of RUNX2, Col I, OSX and OPN in the normoxic or hypoxic group on day 3 were quantified, (I) the corresponding representative western blotting images of which were shown. Results are presented as the means ± SD from ≥ three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. FUNDC1, FUN14 domain-containing 1; Nor, normoxia; Hypo, hypoxia; NC, negative control; si, small interfering; ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; Col I, collagen type I; OSX, osterix; OPN, osteopontin.