TABLE 3.

Primers used in this study.

Name	Primers	Sequences (5’ - 3′)
P1	Trc-SacI-F	CGA​GCT​CTT​GAC​AAT​TAA​TCA​TCC​GGC​TCG
P2	AraDH-BamHI-R	CGG​GAT​CCT​TAC​GGG​GTG​ATA​A
P3	Trc-SpeI-F	CTA​GAC​TAG​TTT​GAC​AAT​TAA​TCA​TCC​GGC​TCG
P4	AraD-KpnI-R	GGG​GTA​CCT​TAA​GAT​TTG​CAT​TTG​TAT​TCT​TCG
P5	Trc-ADG-F	TTT​CTC​CGG​TTA​AAT​AAG​TCT​CCC​TTA​TGC​GAC​TCC​TGC​ATT​AGG
P6	Trc-ADG-R	GGT​CGA​CTC​TAG​AGG​ATC​GGA​TCC​TTA​ACG
P7	Trc-ADG-SacI-F	CGA​GCT​CTT​ATG​CGA​CTC​CTG​CAT​TAG​GAA​ATA​CT
P8	Trc-ADG-BamHI-R	CGG​GAT​CCT​TAA​CGA​CCG​AAA​GCG​TCA​GTA​CC
P9	Trc-ADT-F	TGC​ATT​AGG​AAA​TAC​TAG​ACT​CCT​GCA​TTA​GGA​AAT​ACT​AGT​TTG​ACA​AT
P10	Trc-ADT-R	GGA​TGA​TTA​ATT​GTC​AAG​TTA​GTG​AGA​GTG​ACG​CGG​AAC​TTC​AG
P11	Trc-bdhA-KpnI-F	GGG​GTA​CCT​TGA​CAA​TTA​ATC​ATC​CGG​CTC​G
P12	Trc-bdhA-SalI-R	ACG​CGT​CGA​CAT​TTG​TCC​TAC​TCA​GGA​GAG​C
P13	Trc-ADH2-KpnI-F	GGG​GTA​CCT​TGA​CAA​TTA​ATC​ATC​CGG​CTC​GTA
P14	Trc-ADH2-SalI-R	GCG​TCG​ACA​TTT​GTC​CTA​CTC​AGG​AGA​GCG​T
P15	Target-fucI-F	ATGTGCGTACCTACTGGTCAGTTTTAGAGCTAGAAATAGCAAGTT
P16	Target-fucI-R	TGACCAGTAGGTACGCACATACTAGTATTATACCTAGGACTGAGC
P17	Target-yiaE-F	TAC​CGC​TCG​TCG​GGT​TGT​GGGTT​TTA​GAG​CTA​GAA​ATA​GCA​AGT
P18	Target-yiaE-R	CCACAACCCGACGAGCGGTAACTAGTATTATACACTAGTATTATACCTAGGACTGAGC
P19	Target-ycdW-F	ACGCGTGGATGTTGCCAGAGGTTTTAGAGCTAGGTTTTAGAGCTAGAAATAGCAAGT
P20	Target-ycdW-R	CTCTGGCAACATCCACGCGTACTAGTATTATACCACTAGTATTATACCTAGGACTGAGC
P21	Target-yagE-F	CATGCTGCGCAGGTGGGCGAGTTTTAGAGCTAGTTTTAGAGCTAGAAATAGCAAGT
P22	Target-yagE-R	TCGCCCACCTGCGCAGCATGACTAGTATTATACACTAGTATTATACCTAGGACTGAGC
P23	Target-yjhH-F	CCGCAGAATACCGAAAACGAGTTTTAGAGCTAGTTTTAGAGCTAGAAATAGCAAGT
P24	Target-yjhH-R	TCGTTTTCGGTATTCTGCGGACTAGTATTATACCACTAGTATTATACCTAGGACTGAGC

The underlined part indicates the N20 sequence.