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. 2021 Nov 12;20(4):646–659. doi: 10.1111/pbi.13743

Figure 3.

Figure 3

miR1871 suppresses the expression of MFAP1. (a) Confocal images of YFP‐UTRMFAP1 fluorescence signals suppressed by miR1871. The 35S: YFP‐UTRMFAP1 reporter construct was transiently expressed alone or co‐expressed with 35S: MIR1871 in Nicotiana benthamiana leaves by Agrobacterium‐mediated infiltration at indicated optical density (O.D.) concentrations. Size bars, 100 μm. (b) Confocal images of YFP‐UTRMFAP1 fluorescence signals co‐expressed with miR1871 and MIM1871. The 35S: YFP‐UTRMFAP1 reporter construct was transiently expressed alone or co‐expressed with 35S: MIR1871 and 35S: MIM1871 in N. benthamiana leaves by Agrobacterium‐mediated infiltration at indicated optical density (O.D.) concentrations. Size bars, 100 μm. GV3101 was used to complement the concentration of total bacteria. (c) Western blotting analysis showed the protein amounts of YFP in N. benthamiana leaves in (a). Protein extracted from the infiltrated leaves was subjected to Western blot analysis with anti‐GFP sera. The Ponceau‐stained Rubisco was used as the control indicating the equal sample loading. These experiments were repeated two times with similar results.