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. 2022 Mar 2;50(6):3190–3202. doi: 10.1093/nar/gkac107

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — CTCF localizes to histone marks transitions along the BLV proviral genome. (A) Schematic representation of the BLV proviral genome with the localization of the ChIP-qPCR primers used. Chromatin prepared from BLV infected (B) L267 cells or (C) ovine PBMCs was immunoprecipitated with specific antibodies directed against histone H3 (PanH3), different histone post-translational modifications (H3Kme2, H3Kme3, AcH3, H3K9ac, H3K27ac) or with an IgG as background measurement. Results are presented as histograms indicating percentages of immunoprecipitated DNA compared to the input DNA (%IP/input) normalized to PanH3. Red dashed lines represent CTCF binding sites. Data are the means ± SD from one representative of at least three independent experiments.