Figure 5.

The Rad21 subunit of the cohesin multiprotein complex is recruited to the BLV CTCF binding sites. Chromatin prepared from BLV-infected (A) L267 cell line or (B) YR2 cell line was immunoprecipitated with a specific antibody directed against Rad21. Recovered DNA or non-immunoprecipitated DNA (Input) were then sequenced in paired-end and reads were mapped to a hybrid ovine genome containing the BLV provirus sequence at their respective insertion site and orientation (see Supplementary Table S4 for further details). The orientation of the CTCF motifs is indicated by red triangles.