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. 2022 Feb 25;50(6):3254–3275. doi: 10.1093/nar/gkac133

Figure 1.

Figure 1.

Reduction of hPXR levels leads to an increase in hCAR-dependent CYP2B6 expression in human HepaRG cells. (A, B) HepaRG cells were transfected with non-targeting siRNA (siNT) or pooled siRNA targeting hPXR (siPXR) at 25 nM for 48 h then treated with DMSO or CITCO for an additional 24 h. RT-qPCR was performed for (A) hPXR or (B) CYP2B6 RNA. FC, fold change over siNT and DMSO treated cells. (C) CYP2B6 mRNA levels in parental wild-type (WT) cells compared to those in hPXR KO HepaRG cells treated with DMSO or 1 μM CITCO. FC, fold change over DMSO treated WT cells. (D) Western blot of CYP2B6 and hPXR proteins in WT and hPXR KO HepaRG cells. β-actin was used as the protein loading control. * P < 0.05; *** P < 0.0005.