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. 2022 Mar 7;50(6):3445–3455. doi: 10.1093/nar/gkac158

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Titration curves for oligodeoxynucleotides with d(TCCC)₅ and d(TCCC)₉ repeats. CD spectra for each repeat over the titration range pH 5 to pH 8. In each titration, the characteristics i-motif signature emerge as pH is decreased, with the full signature emerging at pH 5 for d(TCCC)₅ and at pH 5.5 for d(TCCC)₉. Transition pH (pH_T) values show that a significant fraction of each sequence would be present as i-motif near physiological pH.