Table 1.
UV melting and annealing temperatures of d(TCCC) repeats determined by the first derivative method. DNA at 2.5 μM in 10 mM sodium cacodylate with 100 mM KCl at pH 6.5. Values are the mean from triplicate experiments with standard deviations. Transitional pH values determined by CD, with standard errors from the curve fit*
| Sequence* | T m (°C) | T a (°C) | Hysteresis (°C) | pHT |
|---|---|---|---|---|
| d(TCCC)4 | 15.6 ± 1.0 | 13.8 ± 1.2 | 1.8 ± 2.1 | 6.6 ± 0.01 |
| d(TCCC)5 | 28.3 ± 0.6 | 26.0 ± 0.6 | 2.4 ± 1.0 | 6.7 ± 0.04 |
| d(TCCC)6 | 31.4 ± 0.6 | 29.2 ± 0.0 | 2.2 ± 0.6 | 6.8 ± 0.02 |
| d(TCCC)7 | 32.8 ± 1.0 | 29.1 ± 0.0 | 3.7 ± 1.0 | 6.8 ± 0.03 |
| d(TCCC)8 | 40.7 ± 0.6 | 30.7 ± 0.6 | 10.0 ± 0.0 | 6.9 ± 0.01 |
| d(TCCC)9 | 40.7 ± 0.6 | 30.3 ± 0.6 | 10.4 ± 0.6 | 7.1 ± 0.04 |
| d(TCCC)12 | 40.2 ± 0.6 | 30.5 ± 0.0 | 9.8 ± 0.6 | 7.2 ± 0.08 |
| d(TCCC)14 | 42.0 ± 4.0 | 30.4 ± 0.0 | 11.6 ± 4.0 | 7.1 ± 0.07 |
| d(TCCC)15 | 40.2 ± 4.0 | 23.2 ± 2.0 | 16.0 ± 2.0 | 7.1 ± 0.08 |