Figure 2.

In vitro test on the specificity and stability of Sgc8. (A) Binding ability to HCT116 cells and Ramos cells tested with flow cytometric assay. Unmodified Sgc8 was used as the positive control, and library (lib) was used as the negative control. The cells were incubated with DNAs respectively at a concentration of 250 nM in a binding buffer for 30 min. The incubation temperature was 4°C. (B) Stability of Sgc8 variant after incubation with 10% FBS for 48 h analysis by PAGE. (C) Flow cytometric assay of HCT116 cells treated with Sgc8 and Sgc8-F23 after incubation with 10% FBS for 0–36 h, illustrating the binding ability after the stability test.