Figure 3.

Characterization of Sgc8-F23. (A) The binding affinity to HCT116 of Sgc8-F23 and Sgc8 at 4°C. (B) Confocal microscopy fluorescence images of HCT116 cells treated with 500 nM Cy5-labeled Sgc8-F23 (red) in binding buffer at 4°C for 30 min or in culture medium (10% FBS) at 37°C for 1 h. The nuclei were counterstained with Hoechst 33342 (blue); scale bar, 20 μm. (C) Circular dichroism spectroscopy of Sgc8 and Sgc8-F23 in DPBS buffer with 5 mM MgCl₂ at 25°C.