Figure 4.

The binding affinity of Sgc8 derivatives to albumin. (A) Binding affinity analysis of Sgc8 derivatives and Sgc8 with aldehyde/sulfate latex beads coated HSA at room temperature. (B) HSA was immobilized to 3.8 μm 40 mg/ml aldehyde/sulfate latex beads (Thermo Fisher Scientific, Waltham, MA USA), after incubation with sgc8 or Sgc8-F23, the beads were washed to remove excess aptamers. The fluorescence signal of HCT116 and Ramos cells treated with the beads (sgc8 or Sgc8-F23 binding with HSA) was collected by flow cytometer.