Table 5.
Results of analgesic activity following hot plate model and opioid receptors evaluation study.
| Samples | Dose (mg/kg) | Reaction time on hot plate | ||
|---|---|---|---|---|
| 30 min | 60 min | 90 min | ||
| Normal saline | 10 ml/kg | 10.12 ± 0.42 | 10.12 ± 0.57 | 10.12 ± 0.33 |
| Normal saline + NLX | 10 ml/kg + 1 | 10.14 ± 0.71 | 10.14 ± 0.47 | 10.14 ± 0.60 |
| Morphine | 5 | 21.64 ± 0.59 | 23.64 ± 0.70 | 19.64 ± 0.60 |
| Morphine + NLX | 5 + 1 | 14.26 ± 0.94 | 16.60 ± 0.66 | 12.40 ± 0.88 |
| Hp. EtAc | 100 | 19.96 ± 0.05 | 22.26 ± 0.04 | 24.80 ± 0.07 |
| Hp. EtAc + NLX | 100 + 1 | 11.30 ± 0.03 | 13.70 ± 0.09 | 16.60 ± 0.48 |
| Hp. Chf | 100 | 20.42 ± 0.08 | 25.60 ± 0.05 | 28.10 ± 0.55 |
| Hp. Chf + NLX | 100 + 1 | 8.53 ± 0.30 | 9.98 ± 0.59 | 14.40 ± 0.71 |
While after 60 minutes, the standard drug tramadol possesses a significant result (P < 0.01). Hp. Chf and Hp. EtAc fractions displayed dose-dependent result causing 61.01 ± 0.45 (P < 0.001) and 38.45 ± 0.50 (P < 0.01) analgesic effect at the dose of 100 mg/kg. Similarly, after 90 minutes, tramadol again exhibited a significant result (P < 0.01), while tested fraction Hp. EtAc has 33.06 ± 0.33 (P < 0.01)% potential. Likewise, tested fraction Hp. Chf at 100 mg/kg body weight exhibited dose-dependent results as that f the standard drug causing 48.75 ± 0.56 (P < 0.001)% inhibitions.