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Direct inhibition of Drp1 prevents mtDNA damage. Cytb mRNA was quantified by qRT-PCR. HG/Mdiv: HRECs in continuous 20 mM glucose with Mdiv; HG/D-si and HG/SC = Drp1-siRNA or control scrambled RNA transfected HRECs in continuous 20 mM D-glucose; NG-HG and NG-HG/Mdiv: HRECs in 20 mM D-glucose for four days followed by 5 mM glucose, without or with Mdiv, respectively, for four additional days; L-Gl: 20 mM L-glucose. ∗p < 0.05 vs. NG, #p < 0.05 vs. HG.
