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. 2022 Mar 31;2022:1450610. doi: 10.1155/2022/1450610

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Copyright © 2022 Wei Feng and Shuo Han.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

The targeted regulatory relationships between ADAMTS9-AS1/miR-206/ACTB and circFN1/miR-206/ACTB pathways. (a) Schematic diagram of the binding sites of miR-206 and ACTB predicted on the TargetScan website. (b) The results for miR-206 and ACTB verified by dual-luciferase reporter gene assay. (c) The binding sites of ADAMTS9-AS1 and miR-206 predicted using the DIANA-LncBase database. (d) The binding sites of circFN1 and miR-206 predicted using the Starbase database. (e) The results of ADAMTS9-AS1 and miR-206 verified by dual-luciferase reporter gene assay. (f) The results of circFN1 and miR-206 verified by dual-luciferase reporter gene assay. (g) Results of ADAMTS9-AS1 and miR-206 verified by RIP assay. (h) Results of circFN1 and miR-206 verified by RIP assay. (i) Results of ADAMTS9-AS1 and miR-206 observed by FISH. (j) Results of circFN1 and miR-206 observed by FISH. Measurement data were expressed by mean ± standard deviation. Independent sample t test was conducted for comparisons between two groups. ^∗P < 0.05 vs. the mimic NC group or the IgG group.