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. Author manuscript; available in PMC: 2022 Sep 17.
Published in final edited form as: Nat Immunol. 2022 Mar 17;23(4):632–642. doi: 10.1038/s41590-022-01152-y

Extended Data Fig. 1. FACS gating strategy for isolating naïve CD4+ and CD8+ T cells and schematic experimental workflow.

Extended Data Fig. 1

Representative gating strategy for sorting naïve CD4+ and CD8+ T cells are shown. FACS isolated cells were immediately plated on 96 well round bottom plates coated with anti-CD3 (2 μg/ml) and soluble anti-CD28 (1 μg/ml) in the absence or presence of human IL-27 (100 ng/ml) or IFN-β (500 U/ml).