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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Immunol. 2022 Mar 28;23(4):581–593. doi: 10.1038/s41590-022-01158-6

Extended Data Fig. 8. Lymphangiogenesis does not require IFN-γ.

Extended Data Fig. 8

(a): Representative gating strategy used to characterize IFN-γ dependent regulation of Podoplanin and PDL-1. EAE was induced, and the expression of IFN-γ mediated Podoplanin, PDL-1, and CD31 was analyzed by flow cytometry at day 15 post-immunization at score 3.0 of EAE. Gating strategy taken from a representative wild-type EAE sample.

(b – d): Gating strategy for identifying cpLECs between wild-type healthy (b), wild-type EAE score 3.0 (c), and IFN-γ−/− EAE score 3.0 (d).

(e): Quantitation of the average number of cpLECs between wild-type healthy (b), wild-type EAE (c), and IFN-γ−/− EAE (d) reveal lymphangiogenesis in IFN-γ deficient transgenic mice. n = 5 wild-type healthy mice, 5 wild-type EAE mice, and 4 IFN-γ−/− mice; data are represented as mean ± standard error of the mean; for wild-type healthy vs. wild-type EAE, p = 0.0248, wild-type healthy vs. IFN-γ−/− EAE, p = 0.0062, wild-type EAE vs. IFN-γ−/− EAE, p = 0.5964; one-way ANOVA with Tukey’s post-hoc multiple comparisons test.