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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Immunol. 2022 Mar 28;23(4):581–593. doi: 10.1038/s41590-022-01158-6

Figure 4: Inflamed cpLECs activate naïve 2D2 T cells.

Figure 4:

(a – b): Gating strategy for cpLECs collected from EAE and healthy control mice were co-cultured for 24 h with 2D2 T-cells. (a). Quantification of percent of activated (CD69+) 2D2 T-cells after 24 h of co-culture (b). n = 3 experimental replicates, pooled from 4 healthy and 4 EAE mice; data are represented as mean ± standard error of the mean. p = 0.0006; unpaired Student’s t-test.

(c – d): Gating strategy for cpLECs collected from EAE and healthy control mice were co-cultured for 72 h with 2D2 T-cells. (c). Quantification of percent of proliferated (CellTrace Violet) 2D2 T-cells after 72 h of co-culture (d). n = 3 experimental replicates, pooled from 4 healthy and 4 EAE mice; data are represented as mean ± standard error of the mean. p = 0.0092; unpaired Student’s t-test.