Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| AAV9-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 | Mardinly et al., 2018 | N/A |
| AAV9-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 | This article | N/A |
| AAV9-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3 | This article | N/A |
| AAV9-CAG-DIO-ChRmine-ST-P2A-H2B-mRuby3 | This article | N/A |
| AAV9-CAG-DIO-ChrimsonR-ST-P2A-H2B-mRuby3 | This article | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: CD1 (ICR) | Charles River | RRID: MGI:5659424 |
| Mouse: B6;CBA-Tg(Camk2a-tTA)1Mmay/J | Jackson Laboratory | RRID: IMSR_JAX: 003010 |
| Mouse: B6;DBA-Tg(tetO-GCaMP6s)2Niell/J | Jackson Laboratory | RRID: IMSR_JAX: 024742 |
| Mouse: B6.129S2-Emx1tm1(cre)Krj/J | Jackson Laboratory | RRID: IMSR_JAX: 005628 |
| Recombinant DNA | ||
| pCAG-ChroME-mRuby2-ST | Mardinly et al., 2018 | Addgene_108902 |
| pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 | Mardinly et al., 2018 | Addgene_108912 |
| pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST | Pégard et al., 2017 | Addgene_105448 |
| pCAG-Chronos-mRuby2-ST | Pégard et al., 2017 | Addgene_105446 |
| pCAG-ChrimsonR-mRuby2-ST | Pégard et al., 2017 | Addgene_105447 |
| pCAG-ChR2-mRuby2-ST | Mardinly et al., 2018 | Addgene_109125 |
| pCAG-ChroME(F243Y)-mRuby2-ST | Mardinly et al., 2018 | Addgene_109127 |
| pCAG-ChroME2s-mRuby2-ST | Mardinly et al., 2018 | Addgene_109128 |
| pCAG-CoChR-mRuby2-ST | Mardinly et al., 2018 | Addgene_109136 |
| pCAG-ChroME2f-mRuby2-ST | This article | Addgene_170160 |
| pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 | This article | Addgene_170163 |
| pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3 | This article | Addgene_171150 |
| pCAG-ChroMD-mRuby2-ST | This article | Addgene_171158 |
| pCAG-ChRmine-mRuby2-ST | This article | N/A |
| pAAV-CAG-DIO-ChRmine-ST-P2A-H2B-mRuby3 | This article | N/A |
| pCAG-PsChR-mRuby2-ST | This article | N/A |
| pCAG-GtACR1-mRuby2-ST | Mardinly et al., 2018 | Addgene_109136 |
| pFUGW-hGtACR2-EYFP | Govorunova et al., 2013 | Addgene_67877 |
| pAAV-ReaChR-mCherry | This article | N/A |
| Software and algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Cell Profiler | Carpenter et al., 2006 | https://cellprofiler.org/ |
| MATLAB | MathWorks | RRID: SCR_001622 |
| Python | Python Software Foundation | RRID: SCR_008394 |
| Suite2p | Pachitariu et al., 2016 | RRID: SCR_016434 |
| ScanImage | Vidrio Inc. | RRID: SCR_014307 |
| Adobe Illustrator | Adobe | RRID: SCR_010279 |
| Other | ||
| 1p excitation – Spectra X light engine | Lumencor | https://lumencor.com/products/spectra-x-light-engine |
| Tunable laser – Insight X3 | Spectra-Physics | https://www.spectra-physics.com/f/insight-x3-tunable-laser |
| 2p stimulation laser – Femtotrain | Spectra-Physics | https://www.spectra-physics.com/f/femtotrain-femtosecond-oscillator |
| 2p stimulation laser -Monaco | Coherent | https://www.coherent.com/lasers/ultrashort-pulse/monaco |
| 2p stimulation laser- Femtosecond | Satsuma HP2, Amplitude Systems | https://amplitude-laser.com/products/femtosecond-lasers/satsuma/ |
| 2p imaging laser – Mai Tai | Spectraphysics | https://www.spectra-physics.com/f/mai-tai-ultrafast-laser |