Fig. 2.

Protein expression of SAMHD1 and SOX11 after gene silencing of SOX11 or SAMHD1. A: The MCL cell line Granta519 was transfected with specific siRNA targeting SOX11 or SAMHD1, and protein levels were measured 24 h post siRNA by western blot, using beta-actin as loading control. B: SAMHD1 was depleted in Granta519, JeKo-1 and JVM-2 cell lines using Vpx treatment for 72 h. Graphs in A and B are averages of three independent replicates, error bars represent standard error of the mean (SEM), t-test *p < 0.05, **p < 0.01, and a representative blot for each is shown. C: Neither cell number nor cell viability was significantly affected by down-regulation of SOX11 or SAMHD1, or SAMHD1 depletion. D: Cyclin D1 protein expression was assessed by western blot after downregulation or depletion of SOX11 or SAMHD1, graphs show averages of 3–5 independent replicates, error bars represent standard error of the mean (SEM), t-test was applied, and a representative blot for each experiment is shown