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. 2022 Feb 9;477(5):1329–1338. doi: 10.1007/s11010-022-04369-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Treatment of TGF-β activates caspase-1 in LX-2 cells. LX-2 cells were treated with 10 ng/ml TGF-β for 10, 30, or 60 min. a Cell lysates were prepared, and protein expression was analyzed by western blotting. GAPDH was used as an internal control. b Relative levels of active caspase-1 were normalized to GAPDH and quantified using ImageJ software. Data are means ± SEM. *p < 0.05 vs. CON; ^#p < 0.05 vs. TGF-β (10 min) (n = 3/group)