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. 2022 Feb 9;477(5):1329–1338. doi: 10.1007/s11010-022-04369-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Exogenous TGF-β treatment increases the expression level of TGF-β in LX-2 cells. LX-2 cells were treated with 10 ng/mL TGF-β for 24 h. Cell lysates were prepared and the protein levels of active IL-1β (a) and TGF-β (b) were analyzed by western blotting. β-actin and GAPDH were used as the internal controls. Representative western blots (upper panel) and densitometric quantification (lower panel) are shown. Data are means ± SEM. **p < 0.01 vs. CON. (n = 3/group)