Table 1. Primers used in the real-time qPCR assay.

Name	Sequence (5’-3’)	Characterization
ITS1 F	TGC GTT CTT CAT CGA TGC GA	Pan-fungal primer (Forward)
ITS4 R	TAA GCG CAA GTC ATC AGC TTG CGT	Pan-fungal primer (Reverse)
ITS1 F	TAA GCG CAA GTC ATC AGC TTG CGT T	Pan-Candida primer (Forward)
5.8S1R	TGC GTT CTT CAT CGA TGC GA	Pan-Candida primer (Reverse)
ITS1 F2	SSC CCC ATT CTT GTC TAC MTY AC	Trichophyton detection (Forward)
ITS R2	AAC GCT CAG ACT GAC AGC TCT TC	Trichophyton detection (Reverse)
MC F	CCT AAG CGG TGG GTG GTT ACT	Microsporum detection (Forward)
MC R	TGA AAG AAC ATA CCG TCT GAG CG	Microsporum detection (Reverse)

The primer sequences shown in this table were previously published¹⁰,¹¹,¹². ITS: internal transcribed spacer, MC F: Microsporum canis forward, MC R: M. canis reverse.