Table 2. Primers used in the nested PCR assay.
| Name | Sequence (5’-3’) | Characterization | |
|---|---|---|---|
| First-round amplification | |||
| ITS1 | TCC GTA GGT GAA CCT GCG G | ITS target primer (Forward) | |
| ITS4 | TCC TCC GCT TAT TGA TAT GC | ITS target primer (Reverse) | |
| Second-round amplification | |||
| M. restricta F | CTT GGT TGG ACC GTC ACT GG | Malassezia restricta detection (Forward) | |
| M. restricta R | AGG CGG ATG CAA AGT GTC TC | M. restricta detection (Reverse) | |
| M. globosa F | CAA TAA GTG TGT CTC TGC GG | Malassezia globosa detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. globosa detection (Reverse) | |
| M. sympodialis F | CGG ACG CAA ACA CGT CTC TG | Malassezia sympodialis detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. sympodialis detection (Reverse) | |
| M. furfur F | CTA CTC GCG TAC AAC GTC TCT G | Malassezia furfur detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. furfur detection (Reverse) | |
| M. pachydermatis F | CTG CCA TAC GGA TGC GCA AG | Malassezia pachydermatis detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. pachydermatis detection (Reverse) | |
| M. obtusa F | ACC CGT GTG CAC ACT GTT GAG | Malassezia obtusa detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. obtusa detection (Reverse) | |
| M. slooffiae F | ACG CAC GCT AAC ACA ACG TG | Malassezia slooffiae detection (Forward) | |
| 5.8S R | TTC GCT GCG TTC TTC ATC GA | M. slooffiae detection (Reverse) | |
The primer sequences shown in this table were previously published13.