Fig. 2. Rodent neonatal cardiomyocytes express functional BKCa channels.
Traces of whole-cell BKCa channel currents were recorded from A rat neonatal cardiomyocytes and D mouse neonatal cardiomyocytes with or without iberiotoxin (100 nM). Neonatal cardiomyocytes B rat and E mouse, membrane currents were activated by 40 ms voltage steps of 20 mV between −70 to +150 mV from resting −70 mV holding potential. The current versus voltage relationships are plotted with or without iberiotoxin. C and F Percentage block of BKCa current in the presence or absence of iberiotoxin. Cells were isolated from ten independent litters from mice and rats, and data are represented as mean ± SEM. P-values were determined by a one-tailed paired student’s t-test; ****≤ 0.00001, *****≤ 0.000001.