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. 2022 Apr 7;13(4):314. doi: 10.1038/s41419-022-04736-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Relative luciferase activities of wild-type (WT) and mutated (MUT) reporter gene vectors of lncRNA-Gm31932 in B16 cells co-transfected with miR-344d-3-5p mimic/miR-344d-3-5p mut. B Principal flowchart of the RNA pull-down experiment. C The miR-344d-3-5p content, which was enriched from the cell lysate by RNA pull down, was detected by RT-qPCR. D The RNA expression level of miR-344d-3-5p was determined in lncRNA-Gm31932 silenced cells. E, F The mRNA expression of miR-344d-3-5p was detected in ATRA- or PB-4-treated B16 cells. G The rescue effect of the miR-344d-3-5p inhibitor on the growth inhibition of B16 cells mediated by silencing lncRNA-Gm31932 was determined by CCK-8 assay. H The rescue effect of miR-344d-3-5p inhibitor on the morphological changes of B16 cells mediated by silencing lncRNA-Gm31932 was observed using a microscope. I The rescue effect of miR-344d-3-5p inhibitor on the cell cycle arrest of B16 cells mediated by silencing lncRNA-Gm31932 was determined by flow cytometry. J Quantitative analysis of cell cycle distribution (n = 6). Data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01 compared with the corresponding control group by Student’s t-test.